Quality Control: Molecular Diagnostics

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Quality Control: Molecular Diagnostics

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*OverviewSample Collection, Transport, and Processing maintain Nucleic acid integrity through these processes Contamination Control establish a unidirectional work flow from a DNA-free area for reagent preparation, to a sample processing area, to area where amplification and detection occur Positive and Negative Controls Reference: CLSI MM3-A (Molecular Diagnostic Methods for Infectious Diseases)

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*Quality Control: Molecular Diagnostics

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*Real Time PCRSAMPLEPRIMERTaq polymeraseAmplified Target NASYBR Green Quantitative Fluorescence +-In

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*Amplification ReactionsAmplification / TimePOSITIVENO AMPLIFICATION

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*Real Time RT-PCR

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*A/H3

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*A/H5

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*Negative result

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*Risks with PCRmost RT-PCR is still done as “in-house” or “home-brew” primers are not necessarily optimized to test positive controls are “in-house” negative controls are “in-house” interpretation of tests need to be done with caution

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Last Updated: 8th March 2018

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