METHODS FOR PRODUCTION OF AMINO ACIDSEXTRACTION: hydrolysis of proteins to isolate amino acids like cys, tyr, leu
CHEMICAL SYNTHESIS: can result in racemic mixture (D and L amino acids), most applications are for L-form sometime DL or D maybe required.
Direct fermentation: MO use carbon sources and produce aa. Carbon like glu, fructose, alkanes, ethanol, glycerol, molasses, starch, methanol etc.
Conversion of metabolic intermediates to amino acids:
Use of enzymes (microbial) or immobilized cells: resting cells, crude cell extracts, immobilized cells can be used.