Unit II Lecture 4
B. Tech. (Biotechnology) III Year V th Semester
EBT-501, Genetic Engineering
Unit IIRestriction modification
Enzymes used in recombinant DNA technology endonucleases, ligases and other enzymes useful in gene cloning
PCR technology for gene/DNA detection, cDNA,
Use of Agrobacterium for genetic engineering in plants
Gene libraries; Use of marker genes.
Cloning of foreign genes: DNA delivery methods -physical methods and biological methods,
Genetic transformation of prokaryotes: Transferring DNA into E. coli –Chemical induction and Electroporation, Gene Transfer in Plants
The Ti plasmid of Agrobacterium tumefaciens is an important tool for transferring genes into plants.
Agro bacterium tumefacians is a bacterium that causes a disease known as crown gall in plants.
Infects plants by transferring its genetic material into plant cell.
Agrobacterium transformation is the most common technique for genetically engineered plantsAgro-bacterium tumefacians Production of Transgenic PlantsMicroprojectile bombardment
Agrobacterium tumefaciens-mediated transformation
Plant cells are totipotent, so an entire plant can be regenerated from a genetically engineered plant somatic cell. Agrobacterium tumifaciens Infection The Ti Plasmid Transformation of Plant Cells by the Ti Plasmid ...